• M Aparna Post Graduate Resident, Department of Public Health Dentistry, A J Institute of Dental Sciences, Mangalore.
Keywords: Antimicrobial activity, Chlorhexidine, Licorice, Streptococcus mutans, Triphala


Aim:To assess the effectiveness of licorice and triphala against streptococcus mutans and to compare the effect of Licorice, Triphala and Chlorhexidine mouthwash.

Materials and Method:A single blinded quasi experimental study was conducted among the 75 study subjects selected from a tertiary care hospital aged 18-30 years after obtaining ethical clearance.  Aqueous extracts of licorice and triphala were prepared by maceration procedure. The mouth rinse was prepared by adding distilled water (15%) and peppermint oil. The study subjects were divided into 3 equal groups. Group A was provided with Licorice mouth wash, Group B with Triphala and Group C with Chlorhexidine and to safeguard against any bias, the examiner was not informed about the group allotment. The subjects were instructed on the procedure of using mouthwash. Baseline saliva sample was collected and inoculated in mitis salivarius agar and incubated at 370C for 24 hours. After which the colony count was counted and recorded. The saliva samples were again collected on the 7th day and analyzed by the same procedure.

Results:R-commander 2.3-0 was used to analyze the data. Licorice, triphala and chlorhexidine groups showed a reduction of 3.36x104, 3.48x104 and 2.6x104 respectively in mean mutans streptococci colony counts. So the mean mutans streptococci colony counts in all three groups decreased significantly. While comparing the antimicrobial effect of licorice, triphala and chlorhexidine, it was concluded that there was no significant difference between the licorice, triphala and chlorhexidine mouthwashes.

Conclusion:The study affirms that both the licorice and triphala mouthwashes have got similar antibacterial property as chlorhexidine. 

Author Biography

M Aparna, Post Graduate Resident, Department of Public Health Dentistry, A J Institute of Dental Sciences, Mangalore.
Post Graduate Resident, Department of Public Health Dentistry


Digra R, Rao NC, Gupta N, Vasi S. Ayurvedic Herbs in Dentistry: Learn How to Manage Oral Health and Tooth Decay with these Modest Herbs? J Orofac Res 2014;4(1):41-45.

Anagha K, Manasi D, Priya L, Meera M. Antimicrobial activity of Yashtimadhu (Glycyrrhiza glabra L.) - A Review. Int.J.Curr.Microbiol.App.Sci 3(1): pp 329-336.

Bajaj N, Tandon S. The effect of Triphala and Chlorhexidine mouthwash on dental plaque,gingival inflammation, and microbial growth. Int J Ayurveda Res. 2011; 2(1): 29–36.

Nagappan N, John J. Antimicrobial Efficacy of Herbal and Chlorhexidine Mouth rinse -A systematic review. Journal of Dental and Medical Sciences. 2(4. PP 05-1.

Sedighinia F, Afshar AS, Soleimanpour S, Zarif R, Asili J, Ghazvini J. Antibacterial activity of Glycyrrhiza glabra against oral pathogens: an in vitro study. Avicenna J. Phytomed. 2(3):118-124.

Hu C-H, He J, Eckert R, Wu X-Y, Li L-N, Tian Y, Lux R, Shuffer JA, Gelman F, Mentes J et al. Development and evaluation of a safe and effective sugar-free herbal lollipop that kills cavity causing bacteria. Int J Oral Sci, 3(1):13-20.

Prakash S, Shelke AU. Role of Triphala in dentistry. J Indian Soc Periodontol 2014;18(2):132–135.

Jain E, Pandey RK, Khanna R.Licorice root extracts as potent cariostatic agents in pediatric practice. J Indian Soc Pedod Prev Dent. 2013 Jul-Sep;31(3):146-52.

Gupta R, Chandrashekar BR, Goel P, Saxena V, Hongal S, Jain M, et al. Animicrobial efficacy of aqueous and ethanolic extracts of triphala on primary plaque colonizers - An in vitro study. J Young Pharm 2014;6(3):7-13.

Kumar DNM, Sindhu P. The antimicrobial activity of azardirachta indica, glycyrrhiza glabra, cinnamum zeylanicum, syzygium aromaticum, accacia nilotica on streptococcus mutans and enterococcus faecalis-An in vitro study. Editorial 5 Original Research. 2011 Jun;23(1):18.

Ahmad I, Mehmood Z, Mohammad F. Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol 1998;62:18393.

Emilson CG. Potential efficiency of Chlorhexidine against mutans streptococci and human dental caries. J Dent Res. 1994;73:628–9.